In cases where sperm cannot be obtained naturally but fertility treatment with intracytoplasmic sperm injection (ICSI) is required, testicular sperm retrieval can be performed.
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Collecting sperm directly from the testicles for Intracytoplasmic Sperm Injection (ICSI) can be done using three primary methods:
1. PESA (Percutaneous Epididymal Sperm Aspiration)
PESA involves collecting sperm directly from the epididymis—the upper part of the testicle where sperm is stored. This procedure uses a small needle (approximately 2 mm in diameter) to aspirate sperm through the skin.
2. TESA (Testicular Sperm Aspiration)
TESA is a method used when sperm cannot be retrieved from the epididymis or when the sperm quantity obtained via PESA is insufficient. It involves using a small needle to aspirate sperm-containing tissue directly from the testicles.
3. TESE (Testicular Sperm Extraction)
TESE involves surgically obtaining sperm by making a small incision in the testicle to remove a small tissue sample. This method is used when sperm cannot be obtained through TESA or when TESA yields insufficient sperm for ICSI treatment.
When is Testicular Sperm Retrieval Necessary?
1. Diagnostic Purposes
When semen analysis shows no sperm (azoospermia), typically due to blockages in sperm ducts or testicular failure, diagnostic tests are conducted to identify the cause. If a blockage is detected, sperm can be obtained directly from the testicles using TESA and subsequently used for external fertilization (IVF). If the cause is testicular failure, only immature sperm may be present, resulting in significantly reduced treatment success rates.
2. Treatment Purposes
Testicular sperm retrieval can also be performed for therapeutic reasons, such as when it is known that the testicles produce sperm or when the male partner previously fathered children but has undergone vasectomy. It may also be an option if a vasectomy reversal is not desired, or if the reversal’s success rate is limited due to an extended period since the vasectomy. Direct testicular sperm retrieval allows selection of genetically intact sperm, thus enhancing pregnancy success rates.
3. Inability to Retrieve Sperm Naturally
In cases where sperm cannot be naturally obtained but fertility treatment through Intracytoplasmic Sperm Injection (ICSI) is required, testicular sperm retrieval methods are utilized to secure viable sperm for successful fertilization.
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